RapamycinFungusV1, Main, Exploration, bibRecord, 000305

Knockdown of ARK5 expression suppresses invasion of ovarian cancer cells.

Identifieur interne : 000305 ( Main/Exploration ); précédent : 000304; suivant : 000306

Knockdown of ARK5 expression suppresses invasion of ovarian cancer cells.

Source :

Molecular medicine reports [ 1791-3004 ] ; 2019.

RBID : pubmed:30720082

Descripteurs français

English descriptors

KwdEn :
- Cell Line, Tumor (MeSH), Cell Movement (genetics), Cell Proliferation (MeSH), Female (MeSH), Gene Knockdown Techniques (MeSH), Gene Silencing (MeSH), Humans (MeSH), Matrix Metalloproteinase 2 (metabolism), Matrix Metalloproteinase 9 (metabolism), Neoplasm Invasiveness (genetics), Ovarian Neoplasms (genetics), Ovarian Neoplasms (metabolism), Ovarian Neoplasms (pathology), Protein Kinases (genetics), Protein Kinases (metabolism), Proto-Oncogene Proteins c-akt (metabolism), Repressor Proteins (genetics), Repressor Proteins (metabolism), Signal Transduction (MeSH), TOR Serine-Threonine Kinases (metabolism).
MESH :
- chemical , genetics : Protein Kinases, Repressor Proteins.
- chemical , metabolism : Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Protein Kinases, Proto-Oncogene Proteins c-akt, Repressor Proteins, TOR Serine-Threonine Kinases.
- genetics : Cell Movement, Neoplasm Invasiveness, Ovarian Neoplasms.
- metabolism : Ovarian Neoplasms.
- pathology : Ovarian Neoplasms.
- Cell Line, Tumor, Cell Proliferation, Female, Gene Knockdown Techniques, Gene Silencing, Humans, Signal Transduction.

Abstract

The aim of the current study was to investigate the effects and the molecular mechanisms of ARK5 in ovarian cancer cell invasion. The plasmid pGCsilencerU6/GFP/Neo‑RNAi‑ARK5 and the control vector with a scramble sequence were transfected into SKOV3 cells to establish ARK5‑deficient SKOV3 cells (siARK5/SKOV3) and a control cell line (Scr/SKOV3), respectively. Reverse transcription‑polymerase chain reaction (RT‑PCR) and Western blot analysis were used to determine the mRNA and protein expression levels of ARK5. Migration and invasion abilities of SKOV3 cells were determined in chemotaxis and invasion assays, respectively. The epidermal growth factor‑1 (EGF‑1)‑induced expression of matrix metallopeptidase (MMP)‑2 and MMP‑9, epithelial‑mesenchymal transition (EMT) and phosphorylation of mechanistic target of rapamycin kinase (mTOR) in siARK5/SKOV3 and Scr/SKOV3 cells were detected by western blot. RT‑PCR and western blot analyses demonstrated that the expression of ARK5 was significantly downregulated in siARK5/SKOV3 cells at the mRNA and protein levels (P<0.01). The migration and invasion abilities of siARK5/SKOV3 cells were markedly decreased compared with Scr/SKOV3 cells (P<0.01). In addition, the results demonstrated that EGF‑1‑induced expression of MMP‑2 and MMP‑9, EMT and phosphorylation of mTOR were suppressed in siARK5/SKOV3 cells as compared with Scr/SKOV3 cells (P<0.01). The current study demonstrated that ARK5 is a critical factor involved in SKOV3 cell invasion and ARK5 increases invasive potential by promoting EMT and activating the Akt‑mTOR‑MMPs pathway.

DOI: 10.3892/mmr.2019.9901
PubMed: 30720082

Affiliations:

République populaire de Chine

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to stream Main, to step Corpus: 000352
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<author><name sortKey="Shi, Weiwei" sort="Shi, Weiwei" uniqKey="Shi W" first="Weiwei" last="Shi">Weiwei Shi</name>
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<author><name sortKey="Chen, Meiling" sort="Chen, Meiling" uniqKey="Chen M" first="Meiling" last="Chen">Meiling Chen</name>
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<author><name sortKey="Shi, Lihong" sort="Shi, Lihong" uniqKey="Shi L" first="Lihong" last="Shi">Lihong Shi</name>
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<author><name sortKey="Wang, Shuxiao" sort="Wang, Shuxiao" uniqKey="Wang S" first="Shuxiao" last="Wang">Shuxiao Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
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<author><name sortKey="Li, Shuwei" sort="Li, Shuwei" uniqKey="Li S" first="Shuwei" last="Li">Shuwei Li</name>
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<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
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<author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name>
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<author><name sortKey="Wang, Xuejian" sort="Wang, Xuejian" uniqKey="Wang X" first="Xuejian" last="Wang">Xuejian Wang</name>
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<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
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<author><name sortKey="Fang, Chunyan" sort="Fang, Chunyan" uniqKey="Fang C" first="Chunyan" last="Fang">Chunyan Fang</name>
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<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
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<author><name sortKey="Zhang, Baogang" sort="Zhang, Baogang" uniqKey="Zhang B" first="Baogang" last="Zhang">Baogang Zhang</name>
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<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
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<author><name sortKey="Sun, Xiuning" sort="Sun, Xiuning" uniqKey="Sun X" first="Xiuning" last="Sun">Xiuning Sun</name>
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<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, Weifang Medical University, Weifang, Shandong 261053</wicri:regionArea>
<wicri:noRegion>Shandong 261053</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Ruifang" sort="Li, Ruifang" uniqKey="Li R" first="Ruifang" last="Li">Ruifang Li</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Department of Microbiology, Weifang Medical University, Weifang, Shandong 261053</wicri:regionArea>
<wicri:noRegion>Shandong 261053</wicri:noRegion>
</affiliation>
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<author><name sortKey="Shi, Weiwei" sort="Shi, Weiwei" uniqKey="Shi W" first="Weiwei" last="Shi">Weiwei Shi</name>
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<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
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<wicri:noRegion>Shandong 261053</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Meiling" sort="Chen, Meiling" uniqKey="Chen M" first="Meiling" last="Chen">Meiling Chen</name>
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<wicri:regionArea>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053</wicri:regionArea>
<wicri:noRegion>Shandong 261053</wicri:noRegion>
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<author><name sortKey="Shi, Lihong" sort="Shi, Lihong" uniqKey="Shi L" first="Lihong" last="Shi">Lihong Shi</name>
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<term>Cell Movement (genetics)</term>
<term>Cell Proliferation (MeSH)</term>
<term>Female (MeSH)</term>
<term>Gene Knockdown Techniques (MeSH)</term>
<term>Gene Silencing (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Matrix Metalloproteinase 2 (metabolism)</term>
<term>Matrix Metalloproteinase 9 (metabolism)</term>
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<term>Proto-Oncogene Proteins c-akt (metabolism)</term>
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<term>TOR Serine-Threonine Kinases (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Extinction de l'expression des gènes (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>Humains (MeSH)</term>
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<term>Lignée cellulaire tumorale (MeSH)</term>
<term>Matrix metalloproteinase 2 (métabolisme)</term>
<term>Matrix metalloproteinase 9 (métabolisme)</term>
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<term>Transduction du signal (MeSH)</term>
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<term>Tumeurs de l'ovaire (métabolisme)</term>
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<term>Protéines proto-oncogènes c-akt</term>
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</keywords>
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<term>Humans</term>
<term>Signal Transduction</term>
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<term>Femelle</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Prolifération cellulaire</term>
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<front><div type="abstract" xml:lang="en">The aim of the current study was to investigate the effects and the molecular mechanisms of ARK5 in ovarian cancer cell invasion. The plasmid pGCsilencerU6/GFP/Neo‑RNAi‑ARK5 and the control vector with a scramble sequence were transfected into SKOV3 cells to establish ARK5‑deficient SKOV3 cells (siARK5/SKOV3) and a control cell line (Scr/SKOV3), respectively. Reverse transcription‑polymerase chain reaction (RT‑PCR) and Western blot analysis were used to determine the mRNA and protein expression levels of ARK5. Migration and invasion abilities of SKOV3 cells were determined in chemotaxis and invasion assays, respectively. The epidermal growth factor‑1 (EGF‑1)‑induced expression of matrix metallopeptidase (MMP)‑2 and MMP‑9, epithelial‑mesenchymal transition (EMT) and phosphorylation of mechanistic target of rapamycin kinase (mTOR) in siARK5/SKOV3 and Scr/SKOV3 cells were detected by western blot. RT‑PCR and western blot analyses demonstrated that the expression of ARK5 was significantly downregulated in siARK5/SKOV3 cells at the mRNA and protein levels (P<0.01). The migration and invasion abilities of siARK5/SKOV3 cells were markedly decreased compared with Scr/SKOV3 cells (P<0.01). In addition, the results demonstrated that EGF‑1‑induced expression of MMP‑2 and MMP‑9, EMT and phosphorylation of mTOR were suppressed in siARK5/SKOV3 cells as compared with Scr/SKOV3 cells (P<0.01). The current study demonstrated that ARK5 is a critical factor involved in SKOV3 cell invasion and ARK5 increases invasive potential by promoting EMT and activating the Akt‑mTOR‑MMPs pathway.</div>
</front>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30720082</PMID>
<DateCompleted><Year>2019</Year>
<Month>07</Month>
<Day>30</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>07</Month>
<Day>30</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1791-3004</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>19</Volume>
<Issue>4</Issue>
<PubDate><Year>2019</Year>
<Month>Apr</Month>
</PubDate>
</JournalIssue>
<Title>Molecular medicine reports</Title>
<ISOAbbreviation>Mol Med Rep</ISOAbbreviation>
</Journal>
<ArticleTitle>Knockdown of ARK5 expression suppresses invasion of ovarian cancer cells.</ArticleTitle>
<Pagination><MedlinePgn>2927-2934</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3892/mmr.2019.9901</ELocationID>
<Abstract><AbstractText>The aim of the current study was to investigate the effects and the molecular mechanisms of ARK5 in ovarian cancer cell invasion. The plasmid pGCsilencerU6/GFP/Neo‑RNAi‑ARK5 and the control vector with a scramble sequence were transfected into SKOV3 cells to establish ARK5‑deficient SKOV3 cells (siARK5/SKOV3) and a control cell line (Scr/SKOV3), respectively. Reverse transcription‑polymerase chain reaction (RT‑PCR) and Western blot analysis were used to determine the mRNA and protein expression levels of ARK5. Migration and invasion abilities of SKOV3 cells were determined in chemotaxis and invasion assays, respectively. The epidermal growth factor‑1 (EGF‑1)‑induced expression of matrix metallopeptidase (MMP)‑2 and MMP‑9, epithelial‑mesenchymal transition (EMT) and phosphorylation of mechanistic target of rapamycin kinase (mTOR) in siARK5/SKOV3 and Scr/SKOV3 cells were detected by western blot. RT‑PCR and western blot analyses demonstrated that the expression of ARK5 was significantly downregulated in siARK5/SKOV3 cells at the mRNA and protein levels (P<0.01). The migration and invasion abilities of siARK5/SKOV3 cells were markedly decreased compared with Scr/SKOV3 cells (P<0.01). In addition, the results demonstrated that EGF‑1‑induced expression of MMP‑2 and MMP‑9, EMT and phosphorylation of mTOR were suppressed in siARK5/SKOV3 cells as compared with Scr/SKOV3 cells (P<0.01). The current study demonstrated that ARK5 is a critical factor involved in SKOV3 cell invasion and ARK5 increases invasive potential by promoting EMT and activating the Akt‑mTOR‑MMPs pathway.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Shuxiao</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Shuwei</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Physics, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
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<ForeName>Hui</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
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<ForeName>Wei</ForeName>
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<AffiliationInfo><Affiliation>Department of Gynecology, Zhenjiang Maternity and Child Health Hospital, Zhenjiang, Jiangsu 212001, P.R. China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Gao</LastName>
<ForeName>Yuxue</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Xuejian</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fang</LastName>
<ForeName>Chunyan</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Baogang</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Pathology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Xiuning</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Ruifang</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Weiwei</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Meiling</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Lihong</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>01</Month>
<Day>28</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Greece</Country>
<MedlineTA>Mol Med Rep</MedlineTA>
<NlmUniqueID>101475259</NlmUniqueID>
<ISSNLinking>1791-2997</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012097">Repressor Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.7.-</RegistryNumber>
<NameOfSubstance UI="D011494">Protein Kinases</NameOfSubstance>
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<Chemical><RegistryNumber>EC 2.7.1.-</RegistryNumber>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName>
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<MeshHeading><DescriptorName UI="D002465" MajorTopicYN="N">Cell Movement</DescriptorName>
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<MeshHeading><DescriptorName UI="D009361" MajorTopicYN="N">Neoplasm Invasiveness</DescriptorName>
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<MeshHeading><DescriptorName UI="D010051" MajorTopicYN="N">Ovarian Neoplasms</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
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<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
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<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
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<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>03</Month>
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<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Shuxiao" sort="Wang, Shuxiao" uniqKey="Wang S" first="Shuxiao" last="Wang">Shuxiao Wang</name>
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<name sortKey="Chen, Meiling" sort="Chen, Meiling" uniqKey="Chen M" first="Meiling" last="Chen">Meiling Chen</name>
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<name sortKey="Li, Shuwei" sort="Li, Shuwei" uniqKey="Li S" first="Shuwei" last="Li">Shuwei Li</name>
<name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<name sortKey="Shi, Lihong" sort="Shi, Lihong" uniqKey="Shi L" first="Lihong" last="Shi">Lihong Shi</name>
<name sortKey="Shi, Weiwei" sort="Shi, Weiwei" uniqKey="Shi W" first="Weiwei" last="Shi">Weiwei Shi</name>
<name sortKey="Sun, Xiuning" sort="Sun, Xiuning" uniqKey="Sun X" first="Xiuning" last="Sun">Xiuning Sun</name>
<name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name>
<name sortKey="Wang, Xuejian" sort="Wang, Xuejian" uniqKey="Wang X" first="Xuejian" last="Wang">Xuejian Wang</name>
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Knockdown of ARK5 expression suppresses invasion of ovarian cancer cells.

Knockdown of ARK5 expression suppresses invasion of ovarian cancer cells.

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